Rate of Photosynthesis.

BACKGROUND:

Photosynthesis is the process in which light energy is converted to the chemic energy of sugars. It can be summarised in the fol griming equation.

Cardon dioxide + body of water in the presence of chlorophyll and light produces Glucose + Oxygen

This process occurs in the chloroplasts of plant cells which are primarily found in the leaves.

To note the rate of photosynthesis in this practical you are going to criterion the time it takes for click disks to rise to the surface of water in a beaker.

When disks are cut from fresh leaves and kept moist, the cells of the leaf remain alive and are capable of carrying out photosynthesis. usually these leaves would float in a beaker of water but when the parentage is removed from the spongy mesophyll air spaces by creating a low pressure they will sink because they become less expectant than the solution.

In the presence of light the cells in the leaf photosynthesise to produce oxygen which makes them more buoyant until eventually they float to the surface. The quicker the disks rise, the fast the rate of photosynthesis has occurred.

AIM:

To measure the effect of carbon dioxide concentration on the rate of photosynthesis in leaf disks.

MATERIALS REQUIRED:

Fresh green leaves (eg. Ivy)

Cork rock drill or hole punch (approx.



8mm)

Solutions of sodium hydrogen change (2%,4%,6%,8%,10%)

Overhead projector

6 x 25mL Beakers

Sieve

Forceps

Buchner flask and Vacuum pump or a 50 mL plastic syringe

50 mL measuring cylinders

Distilled water

Stop watch

METHOD:

Read done the whole procedure before you begin to allow for the sleepless planning that is necessary. It will help to work in groups of 4 for this experiment.

Beaker Concentration of Hydrogen Carbonate solution

A 0% (Distilled water) B 2% C 4% D 6% E 8%...

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