Beet Cells

MATERIALS AND METHODS

Part I. Temperature stresses on biological membranes.
1.Six (6) 10mm-long beetrootroot outset cores of uniform diameter, using a cork borer.
2.beetroot cores were hardened into a 200mL beaker of tap water (room temp) for 2 minutes. aft(prenominal) 2 minutes gently removed the beet cores and discard the dampen water.
3.Placed apiece beet core into for each one of sextette clean dry test renders. Covered tube with seal material.
4.Placed each one of the test tubes into different climates. render tubes were labeled before being put into the climate. maven in a freezer at or so -20 degrees Celsius, iodine in a refrigerator at active 4 degrees Celsius, One in a water john at about 20 degrees Celsius, One in a water bath at about 45 degrees Celsius, One in a water bath at about 60 degrees Celsius, and One in a water bath at about 70 degrees Celsius. Leaving each test tube in the specified climate for 20 minutes.
5.After the treatment design was over 10mL of distilled water (room temp) was added to each test tube. Beet cores were allowed to sneak for an additional 20 minutes.
6.After 20 minutes, removed beet cores from distilled water.
7.Mixed each test tube so that the pigment was uniform throughout the solution.
8.



Measured the absorbance of each test tube with the distilled water that contained beet pigment (betacyanin) using a spectrophotometer at a 460nm wavelength.
9.Data collected was recorded in lab notebook and also recorded on the blackboard.

Part II. primitive solvent stress on biological membranes.
1.Five (5) 10mm-long beet root cores of uniform diameter, using a cork borer.
2.Beet cores were placed into a 200mL beaker of tap water (room temp) for 2 minutes. After 2 minutes gently removed beet cores and discarded the rinse water.
3.Placed each beet core into each of five clean dry test tubes.
4.Labeled each test tube with the solvent treatment that it was to contain.
a.1% acetone
b.50% acetone
c.1% methanol
d.50% methanol
e.Normal saline
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